Fig. 2

Ectopic CYGB inhibited breast cancer cell proliferation. a Validation of CYGB mRNA overexpression by qRT-PCR. β-actin was detected as an input control. b Validation of CYGB protein overexpression by Western blot. β-actin was detected as an input control. c, d, e and f Ectopic CYGB expression hindered MCF7 and MB231 growth. MCF7 and MB231 cells stably transfected with CYGB or empty vector were used for MTS assay and colony formation assay with and without soft agar to measure proliferation rates. **: p < 0.01 ***: p < 0.001. Scale bar in D indicates 100 μm