Fig. 3

CYGB induced cell cycle arrest, apoptosis, and decreased motility in MCF7 and MB231 cells. a Left, distribution of cell population in various cell cycle stages. Right, quantification of cell cycle distribution. Transiently transfected MCF7 and MB231 cells were stained with propidium iodide (PI) and analyzed by flow cytometry. b Quantification of acridine orange/ethidium bromide (AO/EB) apoptosis staining of transiently transfected MCF7 and MB231 cells. c Left, representative image of transwell migration assay conducted with stably transfected MCF7 and MB231 cells. Right, quantification of migration assay by counting the number of migrated cells/field. d Left, representative image of transwell invasion assay conducted with stably transfected MB231 cells. Right, quantification of invasion assay by counting the number of invaded cells/field. **: p < 0.01 ***: p < 0.001