Fig. 5

Expression of p53 and its downstream targets in CYGB-transfected MCF7 and MB231 cells. a mRNA expression detected by qRT-PCR. b Protein expression detected by Western blot. c p53 protein stability assay. Total protein was extracted from the cells after cycloheximide (CHX, 10 μg/mL) treatment for the indicated time periods. The indicated proteins were detected by Western blot