Table 1 List of primers used in this study

From: The epigenetically downregulated factor CYGB suppresses breast cancer through inhibition of glucose metabolism

PCR	Primer	Sequence (5′-3′)	Product size (bp)	PCR cycles	Annealing temperature (°C)
RT-PCR	CYGB-F	CTTCAGCCAGTTCAAGCACA	194	35	55
	CYGB-R	AGTACACCGGTTCCACCTTG	194	35	55
	GAPDH-F	GGAGTCAACGGATTTGGT	206	23	55
	GAPDH-R	GTGATGGGATTTCCATTGAT	206	23	55
qRT-PCR	HXK2-F	CCAGCTTTTGACCACATTGC	181	40	60
	HXK2-R	GTCTCTGCCTTCCACTCCACT	181	40	60
	TIGAR-F	GCAGCAGCTGCTGGTATAT	241	40	60
	TIGAR-R	GTGTAAACACAGGGCACTCT	241	40	60
	PGM1-F	CAGAGCAGCGCCAACTACG	222	40	60
	PGM1-R	AATGATGCAGGATACAGCAGGG	222	40	60
	GLUT1-F	TCACTGTGCTCCTGGTTCTG	135	40	60
	GLUT1-R	GCTCCTCGGGTGTCTTGT	135	40	60
	β-actin-F	GTCTTCCCCTCCATCGTG	113	40	60
	β-actin-R	AGGGTGAGGATGCCTCTCTT	113	40	60
MSP	bCYGB-M1	GAGGTCGATCGTTAGTTCGTTC	118	40	60
	bCYGB-M2	CAACGACTAACTCGAAAACGCG	118	40	60
	bCYGB-U1	GTGAGGTTGATTGTTAGTTTGTTT	120	40	58
	bCYGB-U2	CCCAACAACTAACTCAAAAACACA	120	40	58

RT-PCR Reverse transcription-PCR, qRT-PCR Quantitative reverse transcription-PCR, MSP Methylation-specific PCR

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