Fig. 1

Immunofluorescence assays of cells comprising the peritoneal microenvironment. a. Clinical samples of peritoneal washes obtained from a cytology-positive case. After TelomeScan was infected at an MOI of 1 for 24 h, and cancer cells were identified as GFP-positive cells, leukocytes and monocytes were stained with A647-labeled anti-CD45 antibodies and PE-labeled anti-CD14 antibodies, respectively. b. Clinical samples of peritoneal wash obtained from another cytology-positive case were analyzed. GFP-positive cells are detected after TelomeScan. The polarity of macrophages to M2 phenotype is confirmed with PE-conjugated anti-CD204