Fig. 2

Induction of the EMT in cancer cells. a. Induction process in THP-1 cells to M1 or M2 macrophages. b. Western blot analyses of CD80, an M1 macrophage marker, CD204, an M2 macrophage marker, and CD68, a pan-macrophage marker. c. Immunofluorescence staining of M1 and M2 type macrophages. d. Morphological changes after Panc1 and BxPC-3 cells were co-cultured with macrophages. e. Western blot analyses show that Panc1 and BxPC-3 cells co-cultured with macrophages express vimentin and α-SMA proteins but have decreased E-cadherin