Fig. 3

Snail is required for SIRT6-mediated aggressive phenotype of NSCLC. a Western blot analysis of Snail protein in A549 and H1299 cells transfected with indicated constructs. One representative experiment of three independent experiments are shown. Numbers indicate fold change in Snail protein. b Wound-healing assay was used to assess the migration of cells transfected with indicated constructs. Data shown are mean from three independent experiments performed in triplicate. ^*P < 0.05. c Transwell invasion assay was used to assess the invasiveness of cells transfected with indicated constructs. Data shown are mean from three independent experiments performed in triplicate. ^*P < 0.05. d The migration and (e) invasion of cells transfected with indicated constructs were determined. Data shown are mean from three independent experiments performed in triplicate. ^*P < 0.05. f Western blot analysis of E-cadherin and vimentin protein levels in cells transfected with indicated constructs after TGF-β1 treatment. One representative experiment of three independent experiments are shown