Fig. 1

BIRC3 expression is upregulated by Fn infection in CRC cell lines. a Hierarchical clustering of the differentially expressed gene heatmap between HCT116 cells and Fn-infected HCT116 cells using Microarray analysis. These genes were affected by changes > 2-fold with p values below 0.05. BIRC3 was the gene with the highest expression (12-fold increase) induced by Fn infection (n = 3). b The relative mRNA expression of the BIRC3 gene determined by qPCR in CRC cell lines infected with or without Fn for 24 h and 48 h respectively. Data are shown as individual values with the median and range from three independent experiments performed in triplicate (n = 6) (***p < 0.001 by unpaired Student t test). c Representative Western blot for BIRC3 protein and GAPDH protein extracted from CRC cells infected with Fn for 0 h, 12 h, 24 h and 48 h