Fig. 2
From: RER1 enhances carcinogenesis and stemness of pancreatic cancer under hypoxic environment
RER1 promotes oncogenicity of PC cells in vitro and in vivo. (a) Overexpression or knockdown of RER1 in Mia PaCa-2 cells were determined by qRT-PCR and Western blotting. (b) Overexpression or knockdown of RER1 in PANC-1 cells were determined by qRT-PCR and Western blotting. (c) CCK-8 assay showed cell survival of RER1 overexpression or knockdown Mia PaCa-2 cells. (d) CCK-8 assay showed cell survival of RER1 overexpression or knockdown PANC-1 cells (e and f) The percentages of BrdU positive Mia PaCa-2 and PANC-1 cells were determined. (g and h) Colony formation assay was conducted to assess cell survival of Mia PaCa-2 and PANC-1 cells (i and j) Cell cycle analysis of Mia PaCa-2 and PANC-1 cells were assessed by flow cytometry after staining with PI. The percentages of cells in three phases are shown in histogram. (k) Tumor growth curve of PANC-1-pSin-vec and PANC-1-pSin-RER1 cells implanted into the flank of male nude mice. (l) Cell proliferation was measured by Ki-67 staining assay. The data represent the mean ± SD from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 (Two-way ANOVA for c and d, student’s t-test for others)