Fig. 1

IATL suppresses cells growth and induces apoptosis in prostate cancer cells. a The chemical structure of IATL. b-c PC-3 and DU145 cells were incubated with increasing doses of IATL (2.5–60 μM) for 24 h respectively. Cell viability was determined by MTT assay. d-g PC-3 or DU145 cells were incubated with IATL for 24 h, percentage of cell apoptosis was determined by Annexin-V/PI staining and flow cytometry. h Cells were incubated with IATL for 20 h, caspase-3 activity in the cell extracts were determined by an assay kit using specific substrate. i-j Cells were incubated with IATL for 20 h, the protein level of cle-caspase-3 was determined by western blot. The results shown are representative of at least three independent experiments. k Cells were incubated with IATL for 20 h, caspase-9 activity in the cell extracts were determined by an assay kit using specific substrate. l-m Cells were incubated with IATL for 20 h, the protein level of Bcl-2 was determined by western blot. The results shown are representative of at least three independent experiments