Fig. 5

Induction of ER stress activation and cancer cell apoptosis by IATL are dependent on ROS production. a-b PC-3 cells were pre-incubated with or without 5 mM NAC for 2 h before exposure to IATL for 4 h. The protein levels of p-eIF2α and ATF4 were determined by western blot. c PC-3 cells were pre-incubated with or without 5 mM NAC for 2 h before exposure to IATL for 6 h. The mRNA expression of CHOP was analyzed by qRT-PCR. d PC-3 cells were pre-incubated with or without 5 mM NAC for 2 h before exposure to IATL for 12 h. The protein level of CHOP was determined by western blot. The results shown are representative of at least three independent experiments