Fig. 6

IATL inhibits STAT3 phosphorylation and expression in prostate cancer cells. a-b The levels of p-STAT3 and STAT3 in PC-3 and DU145 cells were detected by western blot. c-d DU145 cells were treated with IATL (10, 20, or 40 μM) for 12 h, the levels of p-STAT3 and STAT3 were detected by western blot. GAPDH was used as internal control. e-f DU145 cells were pre-incubated with or without 5 mM NAC before exposure to IATL (40 μM) for 12 h, the expression of p-STAT3 and STAT3 were detected by western blot. GAPDH was used as internal control. The results shown are representative of at least three independent experiments