Fig. 3

Combined treatment of DNMTi with HDACi is more effective in bsMCF cells. a Combined treatment of SGI with SAHA or MS275 induces greater inhibition in cell proliferation. b Colony formation in agar methylcellulose and quantification of colonies number. Magnification, 20×. Scale bar, 200 μm. c Quantification graphs at the time point of 6 h for cell migration, and 7 h for invasion by RTCA assay. Cells were first treated and then viable cells were used for both migration and invasion assay. d Representative images of 3D culture in collagen, and quantification of the number and types of masses formed by bsMCF cells. Magnification, 40×. Scale bar, 500 μm. e Representative images of cell apoptosis by immunofluorescence. bsMCF cells were treated with indicated agents, four days later, both floating and adherent cells were collected and cytospun on glass slides. Cells were fixed and stained with cleaved caspase 3 antibody. Magnification, 200×. Scale bar, 20 μm. f Quantification of cell apoptosis. The symbols used for indicating statistical results are as follows: * indicates p < 0.05 when compared to control. ** indicates p < 0.01 when compared to control. a indicates p < 0.01 when compared the combined treatment with single agent. b indicates p < 0.05 when compared the combined treatment with SGI. c indicates p < 0.05 when compared the combined treatment with MS275. This rule applies to other figures too