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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Inhibition of Stearoyl-CoA desaturase 1 reverts BRAF and MEK inhibition-induced selection of cancer stem cells in BRAF-mutated melanoma

Fig. 4

SCD1 expression is able to predict the response of BRAF-mutated-melanoma cells to targeted agents. a-b) AKT and ERK pathways were examined by WB analyses in protein lysates prepared from M14, A375 and M14-R cells treated with BRAF and MEK inhibitors or their combination (panel a) grown in adhesion (2D) and as spheroids (3D) (panel b); c) SCD1 protein expression performed on fixed M14, A375 and M14-R spheroids after 96 h of treatment with BRAF and/or MEK inhibitors by Immunofluorescence analyses. Scale bar 50 μm; d) WB analysis of SCD1 protein expression performed on M14-R and M14 spheroids after 96 h of BRAF and/or MEK inhibitors exposure; e) Immunofluorescence analyses on YAP/TAZ expression were performed on fixed M14, Mel 29 and Mel 66 spheroids after 96 h of exposure to BRAF/MEK inhibitors; Scale bars: 10 μm; f) Western blotting analysis of YAP/TAZ in M14, Mel 29 and Mel 66 spheroids after BRAF/MEK inhibitors exposure; g) YAP/TAZ downstream target ctgf, cyr61, birc5 and tead4 expression in A375, M14, Mel 29 and Mel 66 by qRT-PCR analyses

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