Fig. 3

MiR-103a-3p/miR-382-5p functionally targeted circ-DICER1, and reversed the circ-DICER1-mediated viability, migration and tube formation of GECs. a Cartoon of circ-DICER1 arose from DICER1 gene by scanning DICER1 genomic DNA and circBase. b-c The putative binding sites between circ-DICER1 and miR-103a-3p/miR-382-5p were predicted and the relative luciferase activity was expressed as firefly/renilla luciferase activity. Values are means ± SD (n = 5, each group). **P < 0.01 vs. circ-DICER1 Wt + miR-103a-3p/miR-382-5p (+) NC group. d-g MiR-103a-3p/miR-382-5p was identified in circ-DICER1-RISC complex. Relative expression of circ-DICER1 and miR-103a-3p/miR-382-5p were measured using qRT-PCR. Data represent means ± SD (n = 5, each group). *P < 0.05, **P < 0.01 vs. anti-IgG group, ^#P < 0.05, ^##P < 0.01 vs. anti-Ago2 in control group. h-i The co-effects of circ-DICER1 and miR-103a-3p/miR-382-5p on the viability of GECs were evaluated by CCK-8 assay. Data are presented as the means ± SD (n = 5, each group). **P < 0.01 vs circ-DICER1 (−) NC + miR-103a-3p/miR-382-5p (+) NC group. j-k The co-effects of circ-DICER1 and miR-103a-3p/miR-382-5p on the migration of GECs were evaluated by Transwell assay. Data are presented as the means ± SD (n = 5, each group). **P < 0.01 vs circ-DICER1 (−) NC + miR-103a-3p/miR-382-5p (+) NC group. Scale bar represents 30 μm. l-m The co-effects of circ-DICER1 and miR-103a-3p/miR-382-5p on the tube formation of GECs were evaluated by Matrigel tube formation assay. Data are presented as the means ± SD (n = 5, each group). **P < 0.01 vs circ-DICER1 (−) NC + miR-103a-3p/miR-382-5p (+) NC group. Scale bar represents 30 μm