Fig. 6

The expression of ZIC4 in GECs and ZIC4 regulated the viability, migration and tube formation of GECs by activating Hsp90β expression. a-b The mRNA and protein expressions of ZIC4 in AECs and GECs were evaluated by qRT-PCR and western blot. Data represent means ± SD (n = 5, each group). *P < 0.05, **P < 0.01 vs. AECs group. c ZIC4 regulated the viability of GECs. Values are means ± SD (n = 5, each group). **P < 0.01 vs. ZIC4 (+) NC group; ^##P < 0.01 vs. ZIC4 (−) NC group. d ZIC4 regulated the migration of GECs. Values are means ± SD (n = 5, each group). **P < 0.01 vs. ZIC4 (+) NC group; ^##P < 0.01 vs. ZIC4 (−) NC group. Scale bar represents 30 μm. e ZIC4 regulated the tube formation of GECs (black arrows, tube structures; gray arrows, tube branches). Values are means ± SD (n = 5, each group). **P < 0.01 vs. ZIC4 (+) NC group; ^##P < 0.01 vs. ZIC4 (−) NC group. Scale bar represents 30 μm. f Schematic representation of human Hsp90β promoter region in 3000 bp upstream or 200 bp downstream of transcription start site (designated as + 1). ChIP PCR products for putative ZIC4 binding sites and an upstream region not expected to associate with ZIC4 are depicted with bold lines. g-h The mRNA and protein expressions of Hsp90β were detected by qRT-PCR and western blot after overexpression or silencing of ZIC4. Values are means ± SD (n = 5, each group). **P < 0.01 vs. ZIC4 (+) NC group; ^##P < 0.01 vs. ZIC4 (−) NC group