Table 2 Primers and probes used for RT-qPCR
Primer or Probe | Gene | Sequence (5′- > 3′) or Assay ID |
|---|---|---|
Primer | circ-DICER1 | F: TGTAATAATTGTAGCCAAGTAAATCTCC |
R: AAGTAACCATTTTTCAAAACATTCAAG | ||
P:FAM + TAAAGTTATCGTCTGTTCAGTTCTCATTATGACTTG+BHQ1 | ||
GAPDH | F:GGACCTGACCTGCCGTCTAG | |
R:TAGCCCAGGATGCCCTTGAG | ||
P:FAM + CCTCCGACGCCTGCTTCACCACCT+Eclipse | ||
MOV10 | F: CCATGAGGCACATTGTTACG | |
R:AAGTGCTTCACCACCTGCTT | ||
ZIC4 | F:CTAGCGACAAGCCATACACG | |
R:GTAGCCGAATCGTAGCCAGA | ||
Hsp90β | F:GTGGGTTCAGATGAGGAGGA | |
R:TCTGGTCCAAATAGGCTTGG | ||
Probe | MiR-103a-3p | 000439(Applied biosystems) |
MiR-382-5p | 000572(Applied biosystems) | |
U6 | 001973(Applied biosystems) |