Fig. 2

Osthole induces cell cycle arrest in TNBC cells. a Induction of cycle arrest in TNBC cells was determined by PI staining and flow cytometry. Cells were exposed to osthole for 36 h (MDA-MB-231) or 18 h (BT-549). b The distribution of cell cycle phase in cells challenged with osthole. c Representative histograms from flow cytometric analysis in the three TNBC cells treated with osthole [*P < 0.05 and **P < 0.01 compared to DMSO control]. d MDA-MB-231 and BT-549 cells were exposed to 200 μM osthole for 36 h and 18 h, respectively. Levels of G2/M cell cycle-related proteins MDM-2, Cyclin B1 and CDC2 were determined by western blot. GAPDH was used as internal control. Data representative of 3 independent experiments