Fig. 5

STAT3 overexpression rescued osthole-mediated cytotoxic effects in MDA-MB-231 cells. a Representative western blot showing levels of STAT3 and P-STAT3 in MDA-MB-231 cells following transfection with STAT3 plasmid [Control plasmid = control vehicle vector, STAT3 = STAT3 plasmid]. b Quantification of STAT3 protein levels from panel A. [*P < 0.05 compared to control plasmid (V)]. c STAT3 overexpressing cells and vector control transfected cells were exposed to 200 μM osthole for 48 h, and apoptotic cells were determined by Annexin V/PI staining. d Quantification of annexin V/PI staining showing the percentage of apoptotic cells from panel C [***P < 0.001]. (E) STAT3 overexpressing cells and vector control transfected cells were exposed to 200 μM osthole for 36 h, and representative histograms about G2/M cell cycle phase in cells were determined by flow cytometric analysis [*P < 0.05]