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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Novel role of miR-133a-3p in repressing gastric cancer growth and metastasis via blocking autophagy-mediated glutaminolysis

Fig. 4

Identification of GABARAPL1 as the potential target of miR-133a-3p which is responsible for the autophagy activation and mediating the proliferation and metastasis abilities of GC cells. (a). Bioinfomatic predictions of targeting genes of miR-133a-3p; (b). Wild and mutant sequences of GABRAPL1 and ATG13; (c). The luciferase signal significantly decreased in MKN-45 and BGC-823 cells after co-transfection of miR-133a-3p mimics with GAU-WT (ATU-WT) for 48 h. On the contrary, the luciferase signal of GAU-MT (ATU-MT) did not change upon the inhibition of miR-133a-3p mimics. (d). Western blot demonstrated that either knocking down GABARAPL1 or using HCQ could reverse the promotor role of miR-133a-3p IN on autophagy and EMT; (e). We further detected the negative correlation between the expression of miR-133a-3p and GABARAPL1 by performing qRT-PCR among 50 gastric cancer tissues; (f).CCK8 assay showed that the positive role on GC proliferation rates of miR-133a-3p inhibitors was successfully reversed by GABARAPL1 siRNA and HCQ, scale bar = 200 μm. All data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001

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