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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Novel role of miR-133a-3p in repressing gastric cancer growth and metastasis via blocking autophagy-mediated glutaminolysis

Fig. 7

Glutamine metabolism reveals the specific downstream mechanism of miR-133a-3p mediated autophagy. (a). Western blot assay also showed that using HCQ equals the effect using GLS siRNA and BPTES in the aspect of glutamine metabolism inhibition. Moreover,miR-133a-3p was observed to block glutaminolysis. (b). Immunofluorescence with anti-LC3,anti-GLS and anti-GDH showed the co-activation and co-localization of these three proteins which proves the strong synergy between autophagy and glutaminolysis, scale bar = 5 μm. (c). Edu assay showed that GLS siRNA and BPTES could reversed the positive regulation of LV-miR-133a-3p-IN on GC cell proliferation, scale bar = 100 μm. (d). Transwell assay showed that GLS siRNA and BPTES could reversed the positive regulation of LV-miR-133a-3p-IN on GC cell metastasis, scale bar = 200 μm. (e). Western blot assay demonstrated that blocking glutaminolysis could also prevent the promotion role of LV-miR-133a-3p-IN on EMT. All data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001

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