Fig. 2
Alteration of linc01296 affected the proliferation and migration of CRC cells. a CCK8 assay was carried out to measure the absorbance of transfected CRC cells. b Linc01296 affected colony formation ability of CRC cells. c Edu staining also depicted weaken proliferative ability with low linc01296 expression. Green fluorescence: Edu, blue fluorescence: DAPI. d Immunofluorescence staining of Ki67 was obtained by a fluorescence microscope after silinc01296 or vector treatment. Red fluorescence: Ki67, blue fluorescence: DAPI. e Weaken aggressiveness was seen in SW620 cells transfected with silinc01296 than the cells transfected with siSCR. f The viability of CRC cells transfected with silinc01296 was determined by CCK8 assay. g Enhanced linc01296 facilitated colony formation. h Edu staining proved high proliferation of CRC cells. Green fluorescence: Edu, blue fluorescence: DAPI. (I) Ki67 expression in the transfected CRC cells was observed by immunofluorescence. j Migratory and invasive ability was strengthened in SW480 transfected with linc01296. Data are the means ± SD of triplicate determinants (*P < 0.05)