Fig. 1

Slug interacts with components of the SUMOylation pathway. a Slug was found to interact with Ubc9 and SUMO-1 based on a yeast two-hybrid screen. MST3 was used as a negative control. b-d The association of Slug with Ubc9. Lysates of HEK293T cells transfected with the indicated plasmid (b and c) were used to perform exogenous immunoprecipitation assays. d CL1–5 cell lysate were treated with 1% formaldehyde to performed endogenous immunoprecipitation assays. Cross-linked Slug complexes were analyzed using the indicated antibodies for immunoblotting. β-actin was used as the internal control. e Direct interaction of Slug with the Ubc9 protein. Recombinant proteins, as indicated, were examined via a pull-down assy