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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Hypoxia-induced Slug SUMOylation enhances lung cancer metastasis

Fig. 3

The regulation and components of Slug SUMOylation. a, b Ubc9 influences Slug SUMOylation. Lysates of HEK293T cells transfected with the indicated plasmids or siRNA were analyzed by immunoblotting with the indicated antibodies. c E3 enzymes involved in Slug SUMOylation. Lysates of HEK293T cells transfected with different E3 constructs were analyzed by immunoblotting with the indicated antibodies. β-actin was used as the internal control. d Direct interaction of Slug with PIASy. The recombinant GST and GST–Slug proteins produced from bacteria were shown by Coomassie blue (CB) staining. The products of the in vitro transcription/translation system and the results of the pull-down assay were analyzed by immunoblotting using anti-HA antibodies. e Domain mapping of the interaction between Slug and PIASy. HEK293T cells were cotransfected with Slug fragments of varying lengths. The lysates were examined by immunoprecipitation with anti-Flag antibodies. The results of immunoblotting with the indicated antibodies are shown

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