Fig. 3

Effects of ARNTL overexpression or knocking down on nasopharyngeal carcinoma cell viability and colony formation ability in vitro. a Quantitative RT-PCR assay of ARNTL mRNA expression in SUNE1 and HONE1 cells stably overexpressing ARNTL. b Western blotting assay of ARNTL protein expression in SUNE1 and HONE1 cells stably overexpressing ARNTL. c, d The CCK-8 assay demonstrated that overexpression ARNTL reduced the viability of SUNE1 and HONE1 cells. e The colony formation assay showed that overexpression of ARNTL suppressed colony-forming ability of SUNE1 and HONE1 cells. f Quantitative RT-PCR analysis of ARNTL mRNA expression in SUNE1 and HONE1 cells knocking down by ShRNAs. g Western blotting analysis of ARNTL protein expression in SUNE1 and HONE1 cells knocking down by ShRNAs. h, i The CCK-8 assay demonstrated that knocking down ARNTL promoted the viability of SUNE1 and HONE1 cells. j The colony formation assay showed that knocking down ARNTL enhanced colony-forming ability of SUNE1 and HONE1 cells. * indicated P < 0.05; ** indicated P < 0.01