Fig. 3From: ARNTL hypermethylation promotes tumorigenesis and inhibits cisplatin sensitivity by activating CDK5 transcription in nasopharyngeal carcinomaEffects of ARNTL overexpression or knocking down on nasopharyngeal carcinoma cell viability and colony formation ability in vitro. a Quantitative RT-PCR assay of ARNTL mRNA expression in SUNE1 and HONE1 cells stably overexpressing ARNTL. b Western blotting assay of ARNTL protein expression in SUNE1 and HONE1 cells stably overexpressing ARNTL. c, d The CCK-8 assay demonstrated that overexpression ARNTL reduced the viability of SUNE1 and HONE1 cells. e The colony formation assay showed that overexpression of ARNTL suppressed colony-forming ability of SUNE1 and HONE1 cells. f Quantitative RT-PCR analysis of ARNTL mRNA expression in SUNE1 and HONE1 cells knocking down by ShRNAs. g Western blotting analysis of ARNTL protein expression in SUNE1 and HONE1 cells knocking down by ShRNAs. h, i The CCK-8 assay demonstrated that knocking down ARNTL promoted the viability of SUNE1 and HONE1 cells. j The colony formation assay showed that knocking down ARNTL enhanced colony-forming ability of SUNE1 and HONE1 cells. * indicated P < 0.05; ** indicated P < 0.01Back to article page