Skip to main content
Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: SOX9 is a dose-dependent metastatic fate determinant in melanoma

Fig. 4

Overexpression of SOX9 and NEDD9 restore the oncogenic features of SOX10 KD melanoma cells. qRT-PCR (a) and Western blot (b) analysis for the expression levels of SOX10, SOX9 and NEDD9 in A375M and WM266–4 cell lines treated with the indicated constructs. Data are fold change normalized to scramble control and the average of three independent assays. The red arrow indicates the phosphorylated form of NEDD9. GAPDH serves as a loading control. AlamarBlue (c) and transwell invasion assays (d) of each cell line treated with the indicated constructs. e DAPI images of transwell invasion of melanoma cells treated with the indicated constructs. Scale bars: 100 μM. f Representative images of crystal violet stained A375M and WM266–4 clones subjected to different treatments. g Quantification of the number of A375M and WM266–4 colonies treated with the indicated constructs. Error bars represent mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001

Back to article page