Fig. 4

The effect of X-ray irradiation on Wnt pathway factors. a Western blot demonstrated increased Dab2 and Axin expression in LK cells with X-ray irradiation compared with non-irradiated cells (P < 0.05). Subsequent decreased expression of β-catenin and downstream Wnt pathway factors including cyclin D1, MMP-7 and c-myc were also detected (P < 0.05). The combination of X-ray irradiation and Dab2 siRNA transfection significantly reversed the up-regulation of Dab2 and Axin, as well as the decreased expression of β-catenin and downstream factors of the Wnt pathway (P < 0.05). The combination of X-ray irradiation and Axin siRNA did not influence Dab2 expression but significantly reversed the up-regulation of Axin, as well as the down-regulation of β-catenin and downstream factors of the Wnt pathway (P < 0.05); b The histogram of A; c X-ray irradiation did not influence the expression of Dab2 or Wnt pathway factor expression levels in irradiated SPC cells compared with non-irradiated cells, however, combination of X-ray irradiation and Dab2 siRNA significantly down-regulated Dab2 and Axin expression, and the expression of β-catenin, cyclin D1, MMP-7 and c-myc were up-regulated (P < 0.05). The combination of X-ray irradiation and Axin siRNA did not affect Dab2 expression but significantly down-regulated the expression of Axin, and subsequent up-regulation of β-catenin and downstream factors of the Wnt pathway was observed (P < 0.05); d The histogram of C. * P < 0.05