Fig. 3

IL-17a protein is primarily expressed by neutriphils in GC. a, b Association of CD66b + neutriphils and IL-17a + cells at the invasive margin of GC tissues by immunohistochemical staining. Magnifications: × 200. c Analysis of IL-17a and CD66b distribution in GC tissues by immunofluorescence microscope. One of 10 representative micrographs is shown. Magnifications: × 400. d The production of IL-17a in the cell supernatant in the gastric cell lines (GES-1, MKN45 and MK74), the production of IL-17a in the cell supernatant in the neutrophils isolated from GC tissues and nontumoral tissues, neutrophils activated by TTCS and NTCS, the production of IL-17a in the co-culture system were quantified 24 h after change the culture medium by ELISA. * P < 0.05. e the expression level of IL-17a mRNA in the GC cells (MKN45 and MK74), GC cells co-cultured neutrophils, neutrophils, and neutrophils co-cultured GC cells were quantified 24 h after change the culture medium by qRT-PCR. ** P < 0.001