Fig. 2

In vivo evidence for CPT inhibits OS growth. a-b 143B cell-derived tumors were developed in nude mice and treated with vehicle or CPT. Tumor growth was monitored by measuring the tumor volume and tumor weight for 45 days (n = 5 mice/group; **P < 0.01). Representative tumor images are shown here. c Tumors dissected from vehicle and CPT-treated mice were subjected to H&E, Giemsa and Masson’s trichrome (MT) stains. dTumors were subjected to immunohistochemical analysis using Ki-67 and PCNA antibodies. Determination of apoptosis in tumor tissues by TUNEL assay. e Administration of CPT exhibited no toxicity in five major organs. H&E staining was used to evaluate the histology. Bar represents 500 μm