Fig. 3

CPT triggers mitochondrial fragmentation and apoptosis in OS cells. a Representative dot plots of JC-1 aggregates versus JC-1 monomers in response to various CPT treatments of OS cells. b Representative changes in mitochondrial morphology were detected by confocal microscopy at various concentrations of CPT (as indicated). Images of mitochondria (red) and nucleus (blue) were collected by confocal microscope. c Annexin V/PI analysis by flow cytometry followed by CPT treatment (as indicated). The respective cell percentages in early and late apoptosis for different dose treatment are presented in the qantitative analysis. The results were expressed as the means ± SD from three independent experiments. *P < 0.05 and **P < 0.01, significantly different compared with control