Fig. 4

Effect of VERU-111 on invasion and migration of PanCa cells. (A) Effect of VERU-111 on migration of Panc-1 and AsPC-1 cells determine by wound healing assays. The wounded monolayer was incubated in different concentrations of VERU-111 for 24 h. Images of wound healing assays (magnification, × 10) of Panc-1 (i) and AsPC-1 (ii) cells of control and VERU-111 treatment groups after 24 h. (B) Effect of VERU-111 on migration of AsPC-1 and Panc-1 cells using 96-transwell chamber plate. Representative images of migratory Panc-1 and AsPC-1 cells (i) of control and VERU-111 treatment groups after 24 h. Bar graphs (ii) indicating number of migratory Panc-1 and AsPC-1 cells in control and VERU-111 treatment groups. (C) Effect of VERU-111 on invasion of Panc-1 and AsPC-1 cells (i) as determined by Boyden’s Chamber assay. Representative images of control and VERU-111 treatment groups were captured at 20x magnification after 24 h. Bar graphs (ii) indicate number of invaded Panc-1 and AsPC-1 cells. (D) Effect of VERU-111 on real time migration (i) and invasion (ii) of Panc-1 cells using xCELLigence assay. Results are presented as means ± SEM (n = 3). Asterisk (*) denotes the significant value p < 0.05