Fig. 2

GEN alleviated stemness of SKOV3 cells induced by Co-CM. SKOV3 cells with Co-CM from the co-culture of SKOV3-derived OCSLCs with THP-1 macrophages and were treated with or without different concentrations of GEN (10, 20, and 40 μM). The sphere and colony formation rate (a and b, scale bar, 100 μm) and expression levels of CD133 and CD44 (c) as well as Nanog and Oct4 (d) in SKOV3 cells were shown.*P < 0.05, vs SKOV3 cells induced by co-culture were treated with vehicle (0.1% DMSO). ^# P < 0.05, vs SKOV3 cells induced by co-culture treated with GEN (10.0 μM). These experiments were performed in triplicate