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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: RETRACTED ARTICLE: Genistein inhibits stemness of SKOV3 cells induced by macrophages co-cultured with ovarian cancer stem-like cells through IL-8/STAT3 axis

Fig. 5

Effects of alterations of STAT3 expression combined with GEN on M2 polarization of THP-1 macrophage induced by co-culture. The co-culture of SKOV3-derived OCSLCs and THP-1 macrophages expressing shSTAT3 were treated with or without GEN. Ad-GFP: the cells transduced with adenovirus expressing GFP. Ad-shSTAT3: the cells transduced with adenovirus expressing shSTAT3. The levels of CD163 (a), STAT3 and p-STAT3 (b) expression in THP-1 macrophages as well as the contents of IL-10 and IL-12 (c), NO (d), IL-8 (e) in Co-CM induced by co-culture in STAT3 knockdown and GEN alone or in combination were shown. The co-culture of SKOV3-derived OCSLCs and THP-1 macrophages expressing STAT3 were treated with or without GEN. Ad-GFP: The cells transduced with adenovirus expressing GFP. Ad-STAT3: The cells transduced with adenovirus expressing STAT3. The levels of CD163 (f), STAT3 and p-STAT3 (g) expression in THP-1 macrophages as well as the contents of IL-10 and IL-12 (h), NO (I), IL-8 (j) in Co-CM induced by co-culture in STAT3 knockdown and GEN alone or in combination were shown. *P < 0.05, vs treatment with Ad-GFP. # P < 0.05, vs co-treatment with Ad-GFP and GEN (10.0 μM). These experiments were performed at least three times

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