Fig. 6
Effects of alterations of STAT3 expression combined with GEN on stemness of SKOV3 cells induced by Co-CM. SKOV3 cells were treated with Co-CM from co-culture of SKOV3-derived OCSLCs and THP-1 macrophages expressing shSTAT3 were treated with or without GEN. The sphere and colony formation rate (a and b, scale bar, 100 μm) and expression of CD133 and CD44 (c) in SKOV3 cells induced by Co-CM in STAT3 knockdown and GEN alone or in combination were shown. The co-culture of SKOV3-derived OCSLCs and THP-1 macrophages expressing STAT3 were treated with or without GEN. Ad-GFP: The cells transduced with adenovirus expressing GFP. Ad-STAT3: The cells transduced with adenovirus expressing STAT3. The sphere and colony formation rate (d and e, scale bar, 100 μm) as well as the expression of CD133 and CD44 (f) in SKOV3 cells induced by Co-CM in overexpression of STAT3 and GEN alone or in combination were shown. *P < 0.05, vs treatment with Ad-GFP. # P < 0.05, vs co-treatement with Ad-GFP and GEN (10.0 μM). These experiments were performed at least three times