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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: ANKHD1 is required for SMYD3 to promote tumor metastasis in hepatocellular carcinoma

Fig. 1

SMYD3 is associated with poor prognosis of HCC and promotes HCC cells migration and invasion. a IHC analysis of SMYD3 expression in HCC tissues. b Kaplan-Meier analysis of the overall survival or recurrence-free survival in HCC patients according to SMYD3 expression. c Western blot analysis of SMYD3 expression in different HCC cell lines. d) and e Real-time PCR and western blot confirmed the efficiencies of SMYD3 stable overexpression in huh7 cells and stable knockdown in MHCC97H cells, respectively. f and g Wound healing assay, migration assay and invasion assay were performed in SMYD3-overexpressing cells. h and i Wound healing assay, migration assay and invasion assay were performed in SMYD3 knockdown cells. j In vivo lung metastasis assay was used to evaluate the in vivo effects of SMYD3 on tumor metastasis by tail vein injections of cells; representative images of H&E-stained sections were derived from lung metastatic nodules of both groups; Number of metastatic lung foci observed at the surface of the lungs of mice was counted in each group (n = 6). Data are presented as mean ± SD for three independent experiments. *P < 0.05

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