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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: RETRACTED ARTICLE: Endoplasmic reticulum stress triggers Xanthoangelol-induced protective autophagy via activation of JNK/c-Jun Axis in hepatocellular carcinoma

Fig. 3

XAG induces HCC cell autophagy. Cells were co-cultured with 10 and 20 μM of XAG or vehicle for 48 h. a Effect of XAG on the development of acidic vesicle organelles (AVO) in Bel 7402 and SMMC 7721 cells was examined by Acridine orange (AO) staining. Red plot represents AVO. b Western blotting demonstrated expression of autophagy-related proteins, including LC3B-I, LC3B-II, p62/SQSTM1, Beclin-1, and Atg5. GAPDH was used as control group as well. **p<0.01. c Bel 7402 and SMMC 7721 cells were infected with an adenovirus expression of mRFP-GFP-LC3. Effect of XAG on the formation of autolysosome was observed under a fluorescence microscope, with 400× magnification. mRFP was used to label and track the LC3B-II in HCC cells, decrease of GFP indicates the fusion of lysosome and autophagosome, as well as formation of autolysosome. Yellow puncta and red puncta imply to autophagosome and autolysosome, respectively. Results represent independent experiments in triplicate

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