Fig. 6

XAG elevates the phosphorylation levels of JNK and c-jun through ERS pathway. a Bel 7402 and SMMC 7721 cells were treated with 10 and 20 μM of XAG or vehicle for 48 h, and the phosphorylation and total protein level of JNK and c-jun were analyzed by Western blotting. GAPDH was used as control group. **p<0.01. b After cells were pre-treated with or without 2.5 mM of TUDCA (an ERS inhibitor), then were co-cultured with 20 μM of XAG for 48 h, and Western blotting was used to examine the total and phosphorylation level of JNK and c-jun. GAPDH was used as control group as well. **p<0.01. c After cells were transfected with shRNA targeting CHOP, then were treated with or without 20 μM of XAG for 48 h. The expressions of total JNK, total c-jun, p-JNK, p-c-jun were examined by Western blotting. GAPDH was used as control group. **p<0.01