Fig. 2
Ethanol induces STARD10 expression in vivo and in vitro. a Western blotting analysis of STARD10 and ERBB2 expression in ethanol-fed MMTV-neu transgenic mice. Densitometric ratios normalized to actin are shown in the right panel. Results are expressed as fold relative to control (mean ± SE) from 4 mice per group. *p < 0.05 vs. control group. b, c MCF-7 and SKBR-3 cells were plated at density of 0.4 × 106 cells in 6-well/plates and treated with 100 mM ethanol for 48 h. The mRNA levels of STARD10 and ERBB2 in ethanol-treated MCF-7 and SKBR-3 cells were measured by RT-PCR and compared to control. STARD10, ERBB2, pERK, and ERK were analyzed using Western blotting. Results are expressed as fold relative to control (mean ± SE) from 5 experiments. *p < 0.02 vs control left panel; *p < 0.01 vs. control rigth panel