Fig. 3
Ethanol-induced p65 positively regulates STARD10 and ERBB2 expression in MCF-7 and MMTV-neu. a mRNA analysis of STARD10 and ERBB2 was accomplished using RT-PCR in MCF-7 cells. 0.4 × 106 cells were transfected and treated with 100 mM ethanol for 48 h. Western blotting analysis was performed to measure STARD10 and ERBB2 compared to control from 4 indipendent experiments. *p < 0.04 vs. EV. †p < 0.05 vs STARD10 or ERBB2. b mRNA levels of STARD10 and ERBB2 in MCF-7 cells treated with ethanol (100 mM) and transfected with STARD10 overexpression vector and ERBB2 siRNA (10 nM) for 48 h. Results are expressed as fold relative to Sc + EV (mean ± SE) from 3 independent experiments. *p < 0.04 vs.Sc + EV; †p < 0.04 vs. STARD10; ‡p < 0.03 vs. EtOH. c STARD10 and ERBB2 promoter activity analysis in MCF-7 cells using reporter assay from 4 indipendent experiments. *P < 0.04 vs. EV STARD10 promoter. **p < 0.05 vs. EV ERBB2 promoter. d upper panel. RT-PCR analysis of STARD10 and ERBB2 expression. Cells were treated with 100 mM ethanol or p65 transfection. *p < 0.04 vs. EV. Lower panel. Western blotting analysis of STARD10, ERBB2, p65. Results are expressed as fold relative to EV (mean ± SE) from 3 independent experiments. *p < 0.05 vs. EV. e Western blotting analysis of p65 and IkBα in ethanol-treated MMTV-neu mice. Results are expressed as fold relative to control (mean ± SE) from 4 mice per group. *p < 0.05 vs. control tumor