Fig. 6

Ethanol and STARD10 overexpression increase membrane fluidity and intracellular calcium concentration in vitro and in vivo. a Cells were treated with ethanol (100 mM) for 48 h or transfected with StarD10. Membrane fluidity assay in MCF-7 and SKBR-3 cells. Typical epifluorescence microscopic analysis was performed using an excitation filter (350 nm), a dichroic filter (370 nm) and emission filters for monomer fluorescence (405 nm interference filter) and excimer fluorescence (470 nm cut-on filter). The normalized fluorescence was calculated as a ratio of excimer to monomer. b Calcium ion concentrations in cell lysates from ethanol-treated or STARD10 transfected MCF-7 and SKBR-3 cells (100 mM for 48 h). Calcium ion concentration in breast tumor cell lysates from 4 ethanol-fed MMTV-neu transgenic mice. All data are expressed as (mean ± SE) from 3 independent experiments performed in triplicates. *p < 0.05 vs. EV MCF-7 and SKBR-3; *p < 0.04 vs control tumor MMTV-neu