Skip to main content
Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: CXCL12/CXCR4 promotes inflammation-driven colorectal cancer progression through activation of RhoA signaling by sponging miR-133a-3p

Fig. 6

CXCL12/CXCR4-induced lncRNA XIST functions as a ceRNA to regulate Rho A expression by sponging miR-133a-3p. a Schematic of binding sites of miR-133a-3p on lncRNA XIST. b, c HCT116 and SW620 cells were transfected with 200 nM siRNA of CXCR4 for 48 h or overexpressed with CXCR4 with or without 50 ng/μl CXCL12 for 24 h. RT-qPCR was performed to determine the expression of CXCR4 and lncRNA XIST. d, e HCT116 and SW620 cells were transfected with 200 nM siRNA of lnc RNA XIST for 48 h, the expression of RhoA was determined by RT-qPCR and Western blot. f HCT116 cells were transfected with luciferase constructs and miR-133a-3p mimics or inhibitors. The comparison of luciferase activity of wild-type (WT) and mutant (MUT) lncRNA XIST-3’UTR constructs was performed 36 h after transfection. Data was normalized to renilla activity. *P < 0.05 vs. negative control (NC). g RNA immunoprecipitation with anti-Ago2 antibody was applied to evaluate endogenous Ago2 binding to RNA. Western blot was used to confirm the expression of Ago2 in anti-Ago2 immunoprecipitate. The level of lncRNA XIST and miR-133a-3p were analyzed by qPCR from Ago2 precipitate. *P < 0.05 vs. IgG control

Back to article page