Fig. 4
From: Rhein sensitizes human pancreatic cancer cells to EGFR inhibitors by inhibiting STAT3 pathway
Inhibition of STAT3 by rhein blocks EGFR inhibitors activated STAT3. a PANC-1 cells were treated with DMSO (Control), 60 μM rhein, 5 μM erlotinib or the combination of both inhibitors (rhein + erlotinib). Whole-cell protein extracts were analyzed by Western blotting with the indicated antibodies. GAPDH antibody was used as loading control. Figures are representative of three independent experiments. b The PANC-1 cells were treated with DMSO (Control), 60 μM rhein, 2.5 μM afatinib or the combination. c The pancreatic cell lines BxPC-3 were treated with DMSO (Control), 60 μM rhein, 5 μM erlotinib or the combination of both inhibitors (rhein + erlotinib). d The BxPC-3 cells were treated with DMSO (Control), 60 μM rhein, 2.5 μM afatinib or the combination