Fig. 2

NAC diminishes Notch2 depending on lysosomal degradation. a, The mRNA analysis of Notch2 following dose-dependent treatment of NAC. U87 and U251 cells were treated with NAC (5, 10 or 20 mM) for 24 h. β-actin was used as a housekeeping gene. b The western blot analysis of Notch2 under proteasome (MG132, 10 μM) or lysosome (NH₄Cl, 100 μM) inhibition and NAC (10 mM) treatment in U87 and U251 cells. c Notch2 and Itch were examined by western blot in U87 and U251 cells after NAC treatment (10 mM) for 24 h. d and e The western blot analysis of Itch and Notch2 after Itch silencing (d) or after NAC (10 mM) treatment in the presence of si-Itch (e) in U87 and U251 cells. f CRMP5 was analyzed by western blot in U87 and U251 cells after NAC treatment (10 mM) for 24 h. β-actin was used as a loading control. All data are presented as means ± SD of three independent experiments. * P < 0.05 compared with control group, # P < 0.05 compared with NAC group