Fig. 3

Independent of GSH in NAC induces Notch2 decrease. a The ROS levels were analyzed by flow cytometry using DCFH-DA (10 μM) following the treatments. U87 cells were treated with NAC (10 mM), GSH (20 mM) or Ebselen (10 μM) or for 6, 12 and 24 h respectively. b and c The western blot analysis of Notch2 under the treatment as a described. d Total cellular GSH was measured in U87 and U251 cells under pre-treatment of BSO (1 mM, 12 h), followed by NAC (10 mM, 24 h). e Effect of GSH depletion caused by BSO on Notch2 expression using western blot analysis. β-actin was used as a loading control. F and G, Cell viability was analyzed by CCK8 at 450 nm. U87 (f) and U251 (g) cells were treated as d described. All data are presented as means ± SD of three independent experiments. * P < 0.05 compared with control group, # P < 0.05 compared with NAC group