Fig. 2

VLIUS treatments increases HFt-based NPs cell uptake. a SW872 and SW982 cells were plated on poly-l lysine coated slides and the day after incubated for 45 min in the presence of HFt-based NPs at different concentration (0.1–0.25 – 0.5 mg/ml) and following exposed 15 min to VLIUS source with increasing intensities (40–80 – 120 mW/cm²). Control cells were treated 1 h with HFt-based NPs alone. After incubations cells were counterstained with Hoechst to highlight nuclei and analyzed for immunofluorescence dots. Fluorescence dots were quantified as fraction of pixels over the cutoff of 10 using in the green channel of each image. Scale bar is 10 μm. b Histogram reported percentage of uptake and error bars represent the standard deviation of the measured fractions in the images for each experimental condition. Each experiment was carried out in quadruplicate and repeated at least three times. Significance was assessed by using two-way ANOVA and Tukey’s multiple comparisons post-hoc test. * P < 0.05, **P < 0.001, ***P < 0.0001