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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Very low intensity ultrasounds as a new strategy to improve selective delivery of nanoparticles-complexes in cancer cells

Fig. 3

US exposure enhances cellular uptake of LipoplexLUC NP complexes. a HT29 cells plated at density of 5.0 × 105 cells in 60 mm dish, were delivered with lipoplex-LUC cargos and right after exposed to VLIUS source for the indicated times lengths. b HT29 cells plated as reported in A. The day after cells where either delivered with lipoplex-LUC cargos and treated with VLIUS for 15 min (Lipoplex-LUC / VLIUS) or with opposite schedule VLIUS for 15 min and then incubated lipoplex-LUC cargos (VLIUS / Lipoplex-LUC). In all treatments reported in (a and b) cells were collected 48 h after treatments. DNA delivery efficiency was assessed by luciferase assays and values were normalized to protein content and relative LUC activity quantified with respect to control set to 1.0. Each experiment has been repeated three times in triplicate, means and standard deviation of representative experiments are reported. Significance was assessed by one-way ANOVA and Dunnett’s multiple comparisons post-hoc test. *P < 0.05, **P < 0.001, ***P < 0.0001

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