Fig. 5
US treatments increases trabectedin uptake in tumor but not in normal cell. a, b, c and d, respectively 402–91 WT, 402–91 ET, HF and E926 cells were plated at density of 150,000 cells in 60 mm dish, and the day after treated for 1 h with trabectedin at a concentration of 10 or 25 nM. During the treatment cells were also exposed 1, 5, 10 or 15 min to LIUS source at intensities of 80 mW/cm2. Cell vitality was evaluated by Crystal violet assay 48 h after drug removal. Histogram reported percentage of viable cells and error bars represent the standard deviation. Significance was assessed by one-way ANOVA and Dunnett’s multiple comparisons post-hoc test. *P < 0.05, **P < 0.001, ***P < 0.0001