Fig. 2

miR-6875-3p downregulated BTG2 expression via directly targeting its 3’-UTR. a Expression of miR-6875-3p were examined by qRT-PCR in seven cell lines. b, c The protein and mRNA levels of the BTG2 were analyzed after transfection with the miR-6875-3p inhibitor by Western blot and qRT-PCR. d, e The protein and mRNA level of the BTG2 were analyzed after transfection with the miR-6875-3p mimic by Western blot and qRT-PCR. f The predicted sites of miR-6875-3p binding to the 3’-UTR of the BTG2 were detected via bioinformatics prediction tools. The mutated site in the 3’-UTR of the BTG2 is shown. g The effect of miR-6875-3p on luciferase activity induced by the pMIR-BTG2-wt, pMIR-BTG2-mut-1, and pMIR-BTG2-mut-2 reporter plasmids in HL7702 cells was detected via luciferase reporter gene assays. Data are shown as the mean ± SD of three replicates (*p < 0.05)