Fig. 3

The elevated expression of miR-6875-3p promoted the proliferation of HCC cells and tumorigenicity in vivo. a, b miR-6875-3p was down-regulated in Huh7 and BEL7404 cells through the transfection of a miR-6875-3p inhibitor. miR-6875-3p was up-regulated in HL7702 and HepG2 cells via the transfection of a miR-6875-3p mimic. MTT assay was used to analyze the effect of miR-6875-3p on the proliferation of these four cell lines. c, d Huh7 cells stably low-expressing miR-6875-3p or empty vector were injected into the flanks of nude mice. HL7702 cells stably overexpressing miR-6875-3p or empty vector were injected into the flanks of nude mice. Surgically removed tumor tissues from nude mice 5 weeks post-inoculation. Tumor volume at different time points and Tumor weight were measured. e, f Analysis of BTG2 expression in resected tumors tissues by IHC and Western blot. Data are shown as the mean ± SD of three replicates (*p < 0.05)