Fig. 7

miR-6875-3p promoted emergence of stem cell-like behavior in hepatocellular carcinoma cells. a Levels of miR-6875-3p in magnetically sorted CD133⁺ and CD133⁻ were measured by qRT-PCR in HL7702 and Huh7 cells (left). Levels of miR-6875-3p in magnetically sorted EPCAM⁺ and EPCAM⁻ were measured by qRT-PCR in HL7702 and Huh7 cells (right). b Holoclone assays in Huh7 cells transfected with miR-6875-3p inhibitor or empty vector (left) and HL7702 cells transfected with miR-6875-3p mimic or empty vector (right) were used in three experiments (Exp. I, 100 cells/well scored on day 9; Exp. II, 100 cells/well scored on day 13; and Exp. III, 500 cells/well scored on day 7). c SP population in miR-6875-3p-overexpressing and silenced cells was determined by Hoechst 33342 efflux assays. d miR-6875-3p promotes the proliferation, invasion and metastasis of HCC cells via the BTG/FAK/Akt pathway. Data are shown as the mean ± SD of three replicates (*p < 0.05)